Author/Authors :
Leite، Daniela نويسنده Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, Sao Paulo, Brazil , , Morozetti Blanco، Roberta نويسنده Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, Sao Paulo, Brazil , , Cecilia Vieira de Melo، Leyva نويسنده Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, Sao Paulo, Brazil , , Eduardo Fiorio، Cleiton نويسنده Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, Sao Paulo, Brazil , , Moura Martins، Luciano نويسنده Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, Sao Paulo, Brazil , , Mara Ibelli Vaz، Tania نويسنده Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, Sao Paulo, Brazil , , Aparecida Fernandes، Sueli نويسنده Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, Sao Paulo, Brazil , , Tavares Sacchi، Claudio نويسنده Center of Imunology, Instituto Adolfo Lutz, Sao Paulo, Brazil ,
Abstract :
Bordetella pertussis is the causative agent of pertussis. In Brazil, laboratory diagnosis of pertussis is based on the culture. In 2010, was standardized the Real-Time PCR TaqMan® in routine diagnosis. The aim of this study was to evaluate the impact achieved with the introduction of RT-PCR for the routine diagnosis of pertussis and to compare with the results obtained from culture. 4,697 samples of nasopharyngeal secretions collected from suspected pertussis cases and/or contacts were analyzed for RT-PCR and culture, from January 2008 until the end of December 2011. According to the results obtained from the samples 6.9% were culture/RT-PCR positive, 14.8% were positive only for RT-PCR and 0.2% only for culture. Negative samples for both techniques was 3,622 (77.1%) and 1.0% were inconclusive for RT-PCR. The implementation of RT-PCR in routine diagnosis resulted in an increase in laboratory confirmation by almost three times. The RT-PCR assay does not intend to replace the culture technique, but to promote an improvement in the diagnosis of pertussis.
