Author/Authors :
علي نصير، اعظم نويسنده Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan Ali Nasir, A , يونس، محمد نويسنده , , رشيد، آصف نويسنده Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan Rashid, A , عبدالخليق، سيد نويسنده Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan Abdul Khaliq, S , خان، احتشام نويسنده Department of Pathology, College of Veterinary Science, Jhang Campus, Jhang, Pakistan Khan, E , شاه، سجاد حسين نويسنده Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan Shah, S. H , اسلام، آسيم نويسنده Department of Media Sterilization, University of Veterinary and Animal Sciences, Lahore, Pakistan Aslam, A , قومن، منظور احمد نويسنده Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan Ghumman, M. A , جويا، مصرت حسين نويسنده Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan Joiya, M. H
Abstract :
The present investigation was conducted to study the effects of experimental Clostridium perfringens type D enterotoxaemia in teddy goats. Clinical signs started to appear after 30 min of experimental infection like anorexia, diarrhea, dehydration, frothing and dyspnea. Gross lesions consisted of severe congestion in tissues of varying intensity with enlarged mesenteric lymph nodes while histological examination revealed edema of lungs, kidney, and lymph nodes and to some extent in brain along with hemorrhages in lungs and intestines. Clostridium perfringens type D carrying alpha and epsilon toxin genes were amplified with amplicon size about 247 bp and 665 bp, respectively. Human erythrocytes showed the highest hemolysis, 68%, followed by mice, 57%, against culture supernatants. The percentage of hemolysis was significantly higher at 37 C as compared to 25 C except for rabbit and dog.
