Determination of the number of fluorescence emitting molecules in heterogeneous biological objects by use of fluorescence decay function and synchrotron pulse excitation

A way has been suggested to determine the number of fluorescence emitting molecules in heterogenous biological objects. It is based on the measurement of the pre-exponential factor (amplitude) of fluorescence decay function. The method has been illustrated by three series of experiments on the SR beam station of the S-60 synchrotron of the Lebedev Physical Institute.