Author/Authors :
Chakerzehi، Arezu نويسنده Department of Medical Biochemistry, Shahid Sadughi University of Medical Sciences, Yazd, IR Iran Chakerzehi, Arezu , Eivazi Arvanagh، Neda نويسنده Department of Medical Biochemistry, Shahid Sadughi University of Medical Sciences, Yazd, IR Iran Eivazi Arvanagh, Neda , Saedi، Samaneh نويسنده Department of Laboratory Sciences, Faculty of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, IR Iran Saedi, Samaneh , Hematti، Mahdiyeh نويسنده Department of Medical Biochemistry, Shahid Sadughi University of Medical Sciences, Yazd, IR Iran Hematti, Mahdiyeh , Mohiti-Ardakani، Javad نويسنده Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Moradi، Ali نويسنده Department of Medical Biochemistry, Shahid Sadughi University of Medical Sciences, Yazd, IR Iran Moradi, Ali , Shokouhi، Abolfazl نويسنده Department of Medical Biochemistry, Shahid Sadughi University of Medical Sciences, Yazd, IR Iran Shokouhi, Abolfazl
Abstract :
Background: Cancer metastasis is the most important cause of cancer death and different treatment strategies have targeted on preventing the occurrence of metastasis. Quercetin is a flavonoid and widely used as an antioxidant and recent studies have discovered its anticancer and antiproliferative capabilities. Rac1 is protein known to involve in tumor invasion and metastases.
Objectives: In this study, we investigated the effect of quercetin on expression of Rac1 protein.
Materials and Methods: In this experimental study, HeLa cells were treated with quercetin at 2 concentrations (20 and 40 ?M) for 24 hours and then cell lysate was assessed by Western blotting analysis to investigate the impact of quercetin on expression of Rac1 protein.
Results: Quercetin significantly decreased Rac1 expression of HeLa cells assessed by western blotting, in dose-dependent manners (P < 0.05).
Conclusions: This study showed that quercetin decreased Rac1 expression as a marker of metastasis in cervical cancer cells. Therefore, the quercetin may provide an effective new strategy to reduce of tumor metastasis.
