Author/Authors :
Ghafari Khalaf Mohamadi ، S.M نويسنده Department of Marine Biology, Faculty of Marine Sciences and Technologies, Tehran Sciences and Researches Branch, Islamic Azad University , , Shahbazzadeh ، D نويسنده Venom and Toxin Laboratory, Biotechnology Research Center, Pasteur Institute of Iran , , Jamili ، S نويسنده Department of Marine Biology, Faculty of Marine Sciences and Technologies, Tehran Sciences and Researches Branch, Islamic Azad University , , Fatemi، S.M.R. نويسنده Department of Marine Science and Technology, Science and Research Branch, Islamic Azad University. Tehran. Iran , , Pooshang Bagheri ، K نويسنده Venom and Toxin Laboratory, Biotechnology Research Center, Pasteur Institute of Iran ,
Abstract :
Scatophagus argus argus (Green Scat) is a pretty aquarium fish. Its hard spines are venomous and can cause painful injury. In this study 60 specimens of Green Scat were collected periodically from coastal waters of Boushehr (south of Iran) from May 2011 to April 2012. Anatomical features of venomous spines were investigated. Scat venom was extracted from the spines in a new manner for keeping the specimens alive. The nature of venom was tested by SDS-PAGE. Ethical issues and animal welfare principles such as rapid and instantaneous anesthetizing, post operation disinfection and fast recovery of the specimens was practiced in order to minimize the complications. This method enhanced the purity and quantity of venom as demonstrated by 12 separated proteins in electrophoresis. New ethical issues were developed to surviving the specimens and prolong viability as well.
