Author/Authors :
Askari، Mohammad نويسنده Department of Biotechnology, Pasteur Institute of Iran, Tehran, Iran , , Nikpoor، Amin Reza نويسنده Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran. , , Gorjipour، Fazel نويسنده , , Mazidi، Mohsen نويسنده Biochemistry and Nutrition Research Center, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran. , , Sanati، Mohammad Hosein Mohammad Hosein نويسنده Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology (NIGEB). Sanati, Mohammad Hosein Mohammad Hosein , Aryan، Hajar نويسنده Fazeli-Sanati Genetic Laboratory, Tehran, Iran , , Irani، Alireza نويسنده Eye Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran , , Ghasemi-Falavarjani,، Khalil نويسنده , , Nazari، Hossein نويسنده , , Mousavizadeh، Kazem نويسنده Cellular and Molecular Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran. ,
Abstract :
Background: Half of the cases of vision loss in people under 60 years of age have been attributed to age-related macular degeneration (AMD). This is a multifactorial disease with late onset. It has been demonstrated that many different genetic loci are implicated in the risk of developing AMD in different populations. In the current study, we investigated the association of high-temperature requirement A-1 (HTRA1) gene polymorphisms with the risk of developing AMD in the Iranian population.
Methods: Genomic DNA samples were extracted from 120 patients with AMD and 120 healthy age- and sex-matched controls. A 385 base-pair fragment of the HTRA1 gene promoter region was amplified using the polymerase chain reaction (PCR) technique and sequenced. The frequencies of the alleles were calculated and statistical analysis was performed using SPSS software.
Results: Our study demonstrated that the rate of polymorphisms rs11200638 -625 G > A and rs2672598 -487T > C were significantly greater in AMD patients than in healthy controls from the Iranian population.
Conclusions: The results of our study indicate that HTRA1 gene promoter region polymorphisms are associated with the risk of developing AMD in the Iranian population.
