Urease Activity Protection With EDTA Against Nanoparticles (Fe2O3 and Fe3O4) Inactivation

در اين مطالعه اثرات نانو ذرات مغناطيسي Fe2O3 و Fe3O4 و EDTA بر فعاليت urease بررسي شد. اثر نانو Fe2O3 و نانو Fe3O4 بر فعاليت urease بررسي شد. فعاليت urease توسط طيف سنجي نوري UV-Vis در 40 و در 2/7pH= با استفاده از سديم فسفات به عنوان بافر مطالعه شد. اندازه گيري با استفاده از mg/ml075/0 از urease و غلظت نانو Fe2O3 و نانو Fe3O4 بين mg/ml006/0-002/0 انجام شد. مشخص شد كه با افزايش غلظت نانو Fe2O3 و نانو Fe3O4، فعاليت urease كاهش مي يابد. از طرف ديگر، نانو Fe2O3 و نانو Fe3O4 به عنوان يك مهار كننده غير رقابتي براي urease عمل مي كنند. مطالعات حفاظت urease با استفاده از غلظت هاي مختلف EDTA انجام شد (mg/ml008/0-004/0). اين نشان مي دهد كه با افزايش غلظت EDTA، فعاليت آنزيم افزايش مي يابد

In this study the effects of Fe2O3 and Fe3O4 magnetic nanoparticles and EDTA on urease activity was investigated. The effect of nano-Fe2O3 and nano-Fe3O4 on urease activity were investigated. Urease activity was studies by UV-Vis spectrophotometry at 40 °C at pH = 7.2 using sodium phosphate as buffer. Measurements were carried out using 0.075 mg/ml of urease and a range of nano-Fe2O3 and nano-Fe3O4 concentrations between 0.002-0.006 mg/ml. It was found that by increasing the concentration of nano-Fe2O3 and nano-Fe3O4, urease activity will be decreased. On the other hand, nano-Fe2O3 and nano- Fe3O4 act as non competitive inhibitor for urease. Urease protection studies were corried out by using different concentration of EDTA (0.004-0.008 mg/ml). It was shown by increasing the concentration of EDTA, the activity of enzyme increased.