Author/Authors :
-، - نويسنده Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, I.R. Iran Shahinsaz, Leila , -، - نويسنده Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, I.R. Iran Sabahi, Farzaneh , -، - نويسنده Biotechnology Research Center, Pasteur Institute of Iran, Pasteur square, Tehran, I.R. Iran 3Tehran Hepatitis Center, Vesal Avenue, Tehran, I.R. Iran 4Hepatitis Clinic, Special Disease Center, Kerman, IR Iran Karimi, Mohsen , -، - نويسنده Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, I.R. Iran Behzadian, Farida , -، - نويسنده Tehran Hepatitis Center, Vesal Avenue, Tehran, I.R. Iran Alavian, Seyed Moayed , -، - نويسنده Hepatitis Clinic, Special Disease Center, Kerman, IR Iran Zand, Vahid
Abstract :
Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequences corresponding to the Hepatitis delta antigen (HDAg) C-termini were used for genotyping. The results showed that 63.6% (23 of 36) of (HDAb) positive serum samples (as determined by ELISA) were also positive for HDV-RNA. Sequencing and phylogenic analysis of three Iranian HDV isolates revealed the most homology (93%) with an Italian isolate indicating a close relationship and probably a common origin for these isolates.
