Author/Authors :
-، - نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, I.R. Iran Nassiry, Mohammad Reza , -، - نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, I.R. Iran Tahmoorespour, Mojtaba , -، - نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, I.R. Iran Javadmanesh, Ali , -، - نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, I.R. Iran Soltani, Mahdi , -، - نويسنده Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, I.R. Iran Foroutani Far, Saheb
Abstract :
Association of genetic polymorphism in the calpastatin (CAST) gene with average daily gain was examined in Iranian purebred Kurdi sheep. The genotypes for CAST were determined by the PCR-SSCP method. Blood samples were collected from 84 purebred Kurdi sheep belonging to the Kurdi Breeding Station located in the Khorasan province, north-east of Iran. Extraction of genomic DNA was based on the Guanidinium Thiocyanate-Silica gel method. Three genotypes including aa, ab and ac with frequencies of 0.55, 0.32 and 0.13, were observed in this population. Chi-square test confirmed Hardy-Weinberg equilibrium for the CAST loci. Average heterozygosity (37%) of the CAST locus for Kurdi sheep was slightly low. Daily gain birth to weaning (GBW); weaning to six months (GWS); six to nine months (GSN) and nine to yearling (GNY) were analyzed by a statistical model comprising SSCP (Single strand conformation polymorphism) and significant effect (P<0.05) of CAST genotypes was observed for GBW only.
