Author/Authors :
Groach، Rameshwar نويسنده Department of Botany, Kurukshetra University, Kurukshetra 136119 (India) , , Hussain Dar، Muzafar نويسنده Department of Botany, Kurukshetra University, Kurukshetra 136119 (India) , , Chand Badgal، Kartar نويسنده Principal, Govt. Degree College, Hiranagar, Kathua , , Pal، Priyanka نويسنده Department of Botany, Kurukshetra University, Kurukshetra 136119 (India) , , Singh، Narender نويسنده Department of Botany, Plant Tissue Culture Lab., Kurukshetra University, Kurukshetra, 136119 Haryana, India , , Yadav، Kuldeep نويسنده Department of Botany, Plant Tissue Culture Lab., Kurukshetra University, Kurukshetra, 136119 Haryana, India ,
Abstract :
Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental
tree. In vitro callus induction and regeneration from various explants (eaf
segments and cotyledonary leaf) were studied on modified MS medium. The
highest callus induction rate (80%) and multiplication was obtained in 2 mg/l
2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in
efficient shoot regeneration (70%) and development (4.35 shoots). MS half
strength medium supplemented with 0.2 mg/l NAA reported 80% rooting after
21 days of implantation. Mostly, the roots were long and healthy. Plants were
successfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1)
with 65% survival rate under field conditions. The in vitro regenerated plantlets
were hardened and acclimatized successfully.
