Analiza ekspresji genów odpowiedzialnych za rozwój przewlekłej małopłytkowości immunologicznej u dzieci

AbstractBackground lex multifactorial dysregulation of immune system, including cytokines and autoantibodies production, and also proteins modification underlay the primary mechanism of immune thrombocytopenia (ITP). Specific genetic profile of ITP patients is presumed, especially those with a long and complex natural history of the disease. the study xpression analysis of VNN1 and PPARγ in children with immune thrombocytopenia. als and methods ate genes were identified with microarray cDNA procedure. For the validation of VNN1 and PPARγ expression changes we used qRT-PCR performed comparatively in samples of newly diagnosed ITP (ndITP, n = 16), chronic ITP (cITP, n = 8) and patients without thrombocytopenia (n = 5). s lyzed the data of patients, followed for at least 12 months after ITP diagnosis. No significant differences of VNN1 expression profile were found in between groups (p > 0.05). Lower signatures of mean PPARγ normalized expression values were noticed in chronic ITP patients in contrast to newly diagnosed ITP subjects (p = 0.009). Differences between VNN1/PPARγ ratio values found in ndITP group comparing to subjects with progression to cITP were close to statistical significance (p = 0.054). sions is of the VNN1 and PPARγ expression profiles utility in children diagnosed with ITP requires further investigation.