Author/Authors :
Jorfi، Sahand نويسنده Ph.D student of Environmental health Engineering, Tarbiat Modares University, Tehran, Iran. Jorfi, Sahand , Rezaee، Abbas نويسنده , , Jaafarzadeh، Nemat alah نويسنده Department of Environmental Health Engineering, School of Public Health, Environmental Technology Research Center, Ahwaz Jondishapour University of Medical Sciences, Ahwaz, Iran , , Esrafili، Ali نويسنده Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , , Akbari، Hamideh نويسنده Department of Environmental Health, Health Promotion Research Center and, School of Public Health, Zahedan University of Medical Sciences, Zahedan, IR Iran , , Moheb Ali، Ghasem Ali نويسنده 6Research Center of Biotechnology, Research Institute of Petroleum Industry, Tehran, IR Iran ,
Abstract :
Polycyclic aromatic hydrocarbons (PAHs) are persistence organic chemicals with proved carcinogenic and mutagenic hazards. These compounds are usually adsorbed in soils in vicinity of oil and gas industries. Bioremediation of PAHs contaminated soils is difficult due to hydrophobic nature of PAHs. The main purpose of the current study was to determine the pyrene removal efficiency in synthetically contaminated soil, using biosurfactant. Four pure bacterial strains capable of pyrene degradation were isolated from contaminated soils via enrichment techniques. The soil samples were spiked with an initial pyrene concentration of 500 mg/kg and subjected to bioremediation using a mixed culture comprised of previously isolated strains, in addition to application of biosurfactant during 63 days. The pyrene removal efficiency in samples containing biosurfactant, without biosurfactant and controls, were 86.4%, 59.8% and 14%, respectively, after 63 days. The difference of pyrene removal efficiency between the biosurfactant-containing samples and the ones without it was significant (P < 0.05). Application of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa significantly improved pyrene removal in contaminated soils.
