Author/Authors :
Rahimi، Narges نويسنده Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, IR Iran , , Nourani، Mahnaz نويسنده Infection Diseases Research Center, Babol University of Medical Sciences, Babol, IR Iran , , Yahyapour، Yousef نويسنده Infectious Disease and Tropical Center, Babol University of Medical Sciences, Babol, IR Iran , , Kalantar ، Enayatollah نويسنده Envirronmental Health Resaerch Center,KurdistanniversityofMedical Sciences, Sanandaj, Iran Kalantar , Enayatollah , Yasliyanifard، Sommayeh نويسنده Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, IR Iran , , Amin Marashi، Seyed Mahmoud نويسنده Department of Microbiology and Immunology, Alborz University of Medical Sciences, Karaj, IR Iran ,
Abstract :
Cholera is a severe disease which is caused by Vibrio cholerae and it is typically transmitted by either contaminated food or water particularly in developing countries. The most important virulence factor of this bacterium is an enterotoxin called cholera toxin which is a protein complex secreted by the Vibrio cholerae. In this project, we determined the production of cholera toxin at different pH values. Two standard strain of Vibrio cholerae O1 biovar EL Tor N16961 and Vibrio cholerae O1 biovar Classic ATCC 14035 were used. After overnight cultivation of both the strains the total mRNA extracted and converted to total cDNA. By Relative Real-Time PCR analysis the most cholera toxin production in classical and El Tor strains was at pH 8.5 and 8, respectively. Therefore, We may conclude that use of acidic diet will help in reduction of cholera toxin production.
