Author/Authors :
Salimian Rizi، Kobra نويسنده Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran , , Najar-Peerayeh، Shahin نويسنده Department of Bacteriology, Tarbiat Modares University, Tehran, IR Iran , , Bakhshi، Bita نويسنده Department of Medical Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran , , Rahbar، Mohammad نويسنده ,
Abstract :
Salmonellae have become increasingly resistant to antimicrobial agents, partly as a result of genes carried on integrons. Here we describe the antibiotic susceptibility pattern, ESBL production and the prevalence of integrons genes among clinical isolates of Salmonella spp. This descriptive study was done on 110 isolates collected from four hospitals in Tehran during 2012-2013 and identified by routine biochemical tests. Then, disk diffusion method was used for testing the antibiotic susceptibility. ESBL phenotype was confirmed by Combined Disk. The existence of integron classes was investigated by PCR assay through the amplification of integrase genes. Maximal resistance in Salmonella isolates was noticed against trimethoprim–sulfamethoxazole (63/6%) and nalidixic-acid (47/3%). All of isolates were susceptible to imipenem and ciprofloxacin. Four (3.6%) isolates showed ESBLs phenotype. Thirty six of Salmonella isolates have integron but there was not detected class 3 of integrons among isolates. The present study shows the high prevalence of MDR among Salmonella isolates and so alarms the importance of continued monitoring of drug resistance in clinical settings.
