Author/Authors :
Attaran Rezaei، Fatemeh نويسنده Department of Microbiology, Zanjan Branch Islamic Azad University, Zanjan, IR Iran , , Naserpour Farivar، Taghi نويسنده , , Aslanimehr، Masoumeh نويسنده Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, IR Iran , , Shapouri، Reza نويسنده Biology Research Center Zanjan Branch, Islamic Azad University, Zanjan, IR Iran , , Azimi، Akram نويسنده Department of Microbiology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran , , Johari، Pooran نويسنده Cellular and Molecular Research Center, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR Iran ,
Abstract :
Pseudomonas aeruginosa is an opportunistic pathogen mostly affecting hospitalized and immunodeficient patients. Considering the extent of attention paid to the issue of antibiotic resistance and the origin of such resistance and the growing number of treatment failures in patients with burn injury, it seems that investigating the degree of ampC gene expression could be beneficial for the treatment of patients with severe burn injury. The present study focused on identifying different antibiotic patterns, detecting carbapenem resistant Pseudomonas aeruginosa strains isolated from severely burned patients and investigating AmpC gene expression as one of the important mechanisms associated with drug resistance. A total of 189 clinical isolates of carbapenem resistant Pseudomonas aeruginosa, isolated from patients with severe burn injuries, were identified by bacteriological methods followed by determination of their antibiotic resistance patterns by the standard protocol of Kirby–Bauer. The expression of ampC gene was determined by quantitative real-time PCR. Based on our findings, 94.2% of the isolates were resistant to imipenem, 99.5% were resistant to meropenem, and all were resistant to ertapenem. The level of ampC gene expression in carbapenem resistant Pseudomonas aeruginosa isolates, compared to standard carbapenem sensitive strains, was significantly increased. Our data showed that the over-expression of ampC gene is an important mechanism of resistance in carbapenem resistant Pseudomonas aeruginosa strains isolated from severely burned patients with secondary infections.
