Author/Authors :
Ghaemmaghami، Sara نويسنده Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran , , Hedayati، Mehdi نويسنده Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , , Mohaddes، Seyed Mojtaba Seyed Mojtaba نويسنده Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Mohaddes, Seyed Mojtaba Seyed Mojtaba , Gorgian Mohammadi، Masumeh نويسنده Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran , , Barkhordari، Amin نويسنده Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran ,
Abstract :
The plasma concentration of resistin, an adipokine, and its expression are increased significantly in patients with colorectal cancer that suggests its possible role in cancer progression. The aim of this study was to investigate resistin presence and its effect on cell proliferation and telomerase gene expression in colorectal cancer cell line (HCT-116). Resistin and telomerase gene expression in cells were investigated by RT-PCR at mRNA level and the results were confirmed by ELISA method at protein level. The HCT-116 cells were stimulated with resistin. XTT assay was performed to examine cell proliferation. Telomerase gene expression was assessed to increase our knowledge about the possible underlying mechanism on resistin treated cells. The data were obtained from ELISA method, XTT assay, living cells number, and expression level of gene. The statistical analysis was performed with SPSS version15. Resistin was expressed in neither cell lysate nor supernatant. Colorectal cancer cells proliferation was induced by resistin after 24 and 48 hours in a dose-dependent manner (mean ± SD, 0.82 ± 0.0095; P < 0.05). Human telomerase reverse transcriptase (h TERT) gene expression was increased after treating cells with resistin. According to our results, HCT-116 cell is not the main source of resistin in colorectal cancer and high-level plasma concentrations of resistin in colorectal cancer might be due to the increased inflammatory cells in pro-inflammatory state of cancer. Interestingly, resistin promoted colorectal cancer growth via enhancing telomerase expression in a paracrine manner.
