Author/Authors :
Abtahi، Behrooz نويسنده Faculty of Biological Sciences, Shahid Beheshti University G. C., Tehran, Iran , , Mosafer Khorjestan، Saeedeh نويسنده Department of Marine Biology, Faculty of Biological Sciences, Shahid Beheshti University, G.C,Evin, Tehran, IR Iran , , Ghezellou، Parviz نويسنده Department of Marine Biology, Faculty of Sciences, University of Hormozgan, Iran , , Aliahmadi، Atousa نويسنده Department of Biology, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, IR Iran , , Ranaei Siadat، Seyed Omid نويسنده Protein Research Center, Shahid Beheshti University, G.C,Evin, Tehran, IR Iran , , Kazemi، Seyed Mehdi نويسنده Department of Biology, College of Sciences, Qom Branch, Islamic Azad University, Qom, IR Iran , , Ghassempour، Alireza نويسنده , , Fathinia، Behzad نويسنده Department of Biology, Faculty of Sciences, Yasouj University, Yasouj, IR Iran ,
Abstract :
The antibacterial activities of eight snake crude venom (Macrovipera lebetina obtusa, Pseudocerastes persicus, Pseudocerastes urarachnoides, Echis carinatus sochureki, Gloydius halys caucasicus, Naja (naja) oxiana, and two species of true sea snakes Enhydrina schistose and Hydrophis cyanocinctus) were assessed against five important standard pathogenic bacterial strains (Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, vancomycin resistant Enterococcus faecium and Streptococcus pneumoniae). Broth micro-dilution method, standard disk diffusion assay and bio-autography based on polyacrylamide gel electrophoresis were used for this purpose. Snake venom revealed inhibitory effects against S. aureus and E. faecium strains. The moderate effects on S. aureus were showed by venomof P. urarachnoides, P. persicus and N. oxiana with MIC values of 20, 10 and 10 ?g/ml respectively. Moreover, venom of E.carinatus, M.lebetina and G. halys showed strong effects (MICs= 40, 80, 160 ?g/ml) respectively. In the case of E. faecium tested strain, the best inhibitory effects were exhibited by venom of P. persicus, P.urarachnoides, N. oxiana and M. lebetina (MIC= 10 ?g/ml). According to the MIC values and inhibition zones in disk diffusion assays, the strongest antibacterial activity belonged to P. persicus and P. urarachnoides venoms.
