Fluoxetine and diclofenac interaction on food intake in Goldfish, Carassius auratus

This study was carried out to investigate the interaction of simultaneous use of fluoxetine (Flx), a selective serotonin reuptake inhibitor, and diclofenac (Dcf), a non-steroidal anti-in?ammatory drug, on food intake in goldfish, Carassius auratus. Treatments with different dosage of Flx including control, 0, 1, and 10 µg/g body weight (BW) were injected in the fish with mean weight of 30.16 ± 8.57 g every other day in total of 5 times. Then fish were exposed to 3 different levels of Dcf including 0, 10, and 100 mg/l for 5 days. Injection of fluoxetine significantly decreased food intake and consequently body weight. After 5 days exposure to Dcf, the amount of food intake in the Dcf receiving treatments of 1 mg/l and 10 mg/l was significantly larger than that of 0 mg/l Dcf receiving treatment in both the Flx dosage groups of 1 ?g/g BW and 10 ?g/g BW. Our results indicated that Dcf inhibits behavioral change effects of Flx showing the complex effects of pharmaceuticals on fish.