Reconstructing in-situ vegetation dynamics using carbon isotopic composition of biopolymeric residues in the central Chinese Loess Plateau

Carbon isotopic compositions of total organic carbon (TOC) or leaf waxes in soils have been used to reconstruct past changes in the relative abundance of C3 and C4 plants. However, TOC contains organic matter of different source organisms (plants, animals, fungi, bacteria etc.) and its isotopic ratios could be modified during diagenesis since different organic fractions (e.g., carbohydrates, proteins, lipids etc.) have different isotopic ratios and degradation rates. Leaf waxes, on the other hand, are transported by wind and hence represent vegetation input from a large geographic area. We report in this paper a new approach to extract in-situ paleovegetation information from late Quaternary paleosols by determining carbon isotopic compositions of biomarkers derived from aliphatic bio- and geopolymers of plant origin. We show that C9 to C17 n-alkenes derived from aliphatic bio- and geopolymers are the dominant compounds released by flash pyrolysis of insoluble organic matter isolated from the late Quaternary paleosols in the Chinese Loess Plateau. Because the aliphatic polymeric substances are mainly derived from plant leaves, bark and roots, the isotopic signatures of these alkenes, determined using pyrolysis–gas chromatography–isotopic ratio mass spectrometry, reflect in-situ vegetation changes. Our down-core δ13C data from the Xifeng loess–paleosol profile show significant coherence among biopolymer-derived n-alkenes, leaf wax n-alkanes, and TOC for the past 40 kyr. However, the different amplitudes of the temporal isotopic changes among these data sets are indicative of variability in in-situ and regional vegetation dynamics, organic sources and effect of diagenesis. Our data also imply that below-ground biomass is a major source of soil organic matter in the central China loess sediment.