Author/Authors :
-، - نويسنده College of Science, Guangdong Ocean University, Zhanjiang, China Le, Xiaoyan , -، - نويسنده College of Science, Guangdong Ocean University, Zhanjiang, China Luo, Ping , -، - نويسنده College of Science, Guangdong Ocean University, Zhanjiang, China Gu, Yipeng , -، - نويسنده College of Science, Guangdong Ocean University, Zhanjiang, China Tao, Yexing , -، - نويسنده College of Science, Guangdong Ocean University, Zhanjiang, China Liu, Huazhong
Abstract :
Objective(s):Cyclophosphamide (CP) toxicity on testis was hampered by squid ink polysaccharide (SIP) via restoration of antioxidant ability in our previous investigations. This study investigated roles of Nrf2/ARE signal pathway in testis of treated mice.
Materials and Methods: Male Kunming mice were employed to undergo treatment with SIP and/or CP. Protein levels of Nrf2, keap-1, histone deacetylase 2 (HDAC2), quinone oxidoreductase 1 (NQO-1), and heme oxygenase 1 (HO-1) and phosphorylation level of protein kinase C (PKC) in testis were evaluated by Western blotting.
Results: Data showed that SIP elevated expressions of NQO-1 and HO-1 genes, two downstream target molecules of Nrf2, via activating Nrf2 to play preventive roles on CP-treated testis, and further discovered that upstream regulators of Nrf2, keap-1, HDAC2, and PKC, were concerned with the regulation of Nrf2.
Conclusion: These results suggest that SIP could effectively weaken CP-associated testicular damage via Nrf2/ARE signal pathway.
