Author/Authors :
-، - نويسنده Department of Basic Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, Iran|Medical College of Saveh, Saveh University of Medical Sciences, Saveh, Iran Kourosh Arami, Masoumeh , -، - نويسنده Department of Physiology, Tarbiat Modares University, Tehran, Iran Mirnajafi zade, Javad , -، - نويسنده Department of Physiology, Hamedan University of Medical Sciences, Hamedan, Iran Komaki, Alireza , -، - نويسنده Department of Physiology, Kermanshah University of Medical Sciences, Kermanshah, Iran Amiri, Mahmood , -، - نويسنده Department of Basic Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, Iran Mehrpooya, Sara , -، - نويسنده Department of Physiology, Tarbiat Modares University, Tehran, Iran Jahanshahi, Ali , -، - نويسنده Department of Basic Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, Iran Jamei, Behnam
Abstract :
Objective(s): Nucleus Raphe Magnus (NRM) that is involved in the regulation of body temperature contains nitric oxide (NO) synthase. Considering the effect of NO on skin blood flow control, in this study, we assessed its thermoregulatory role within the raphe magnus. Materials and Methods: To this end, tail blood flow of male Wistar rats was measured by laser doppler following the induction of hypothermia. Results: Intra-NRM injection of SNP (exogenous NO donor, 0.1- 0.2 μl, 0.2 nM) increased the blood flow. Similarly, unilateral microinjection of glutamate (0.1- 0.2 μl, 2.3 nM) into the nucleus increased the blood flow. This effectof L-glutamate was reduced by prior intra NRM administrationof NO synthase inhibitor NG-methyl-L-arginine or NG-nitro-L-argininemethyl ester (L-NAME, 0.1 µl, 100 nM). Conclusion: It is concluded that NO modulates the thermoregulatory response of NRM to hypothermia and may interactwith excitatory amino acids in central skin blood flow regulation.
