Author/Authors :
-، - نويسنده Department of Microbiology, School of Science, Alzahra University, Tehran, Iran Shokouhfard, Maliheh , -، - نويسنده Department of Microbiology, School of Science, Alzahra University, Tehran, Iran Kermanshahi, Rouha Kasra , -، - نويسنده Department of Microbiology, School of Science, Alzahra University, Tehran, Iran Vahedi Shahandashti, Roya , -، - نويسنده Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Feizabadi, Mohammad Mehdi , -، - نويسنده Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran Teimourian, Shahram
Abstract :
Objective(s):Serratia marcescens is one of the nosocomial pathogen. The ability to form biofilm is an important feature in the pathogenesis of S. marcescens. The aim of this study was to determine the anti-adhesive properties of a biosurfactant isolated from Lactobacillus acidophilus ATCC 4356, on S. marcescens strains.
Materials and Methods: Lactobacillus acidophilus ATCC 4356, was selected as a probiotic strain to produce biosurfactant. Anti-adhesive activities was determined by pre-coating and co- incubating methods in 96-well culture plates.
Results: The FTIR analysis of derived biosurfactant revealed the composition as protein component. Because of the release of such biosurfactants, L. acidophilus was able to interfere with the adhesion and biofilm formation of the S. marcescens strains. In co- incubation method this biosurfactant in 2.5 mg/ml concentration showed anti-adhesive activity against all tested strains of S. marcescens (P<0.05).
Conclusion: Our results show anti-adhesive properties of L. acidophilus biosurfactant will be useful against microorganisms responsible for infections in the urinary, vaginal and gastrointestinal tracts, as well as skin, making it a suitable alternative to conventional antibiotics.
