Author/Authors :
توكلي، سميه نويسنده , , بنده پور، مژگان نويسنده مرکز تحقيقات سلولي و مولکولي علوم پزشکي-دانشكده پزشكي -دانشگاه عوم پزشكي شهيد بهشتي تهران BANDEH POUR, M , سليماني فر، زينب نويسنده بخش بيوتكنولوژي، دانشكده پزشكي، دانشگاه علوم پزشكي شهيد بهشتي، تهران , , گودرزي، مهدي نويسنده Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Goudarzi, Mehdi , كاظمي ، بهرام نويسنده kazemi, bahram
Abstract :
Background: Influenza virus is the major cause of lower respiratory tract illnesses on the worldwide. Vaccination can be an effective tool to prevent its outbreak. Highly conserved viral nucleoprotein is an effective vaccine candidate to provide heterosubtypic immunity, offering resistance against various influenza virus strains.
Materials and Methods: In present research NP gene was inserted in pET-22b expression vector. New construct (pET-22b/NP) was transformed into E. coli BL21 (DE3) strain and the expression of nucleoprotein was induced by IPTG. It was analyzed by SDS-PAGE and confirmed by Western blotting.
Results: Western blotting confirmed the expression and production of recombinant Influenza nucleoprotein.
Conclusion: These results suggest that the codon-optimized influenza A virus NP gene can be efficiently expressed in E. col
