Author/Authors :
Yildizfer، Ferdag نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey , , Donma، Orkide نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey , , Yen، Mustafa نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey , , Ekmekci، Ozlem نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. , , Asli Karatas Kul، Zubeyde نويسنده Ministry of Health, Diyarbakir Education and Research Hospital, Diyarbakir, Turkey , , Keser، Zafer نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey , , Esat Imal، Ahmet نويسنده Tufts Medical Center, Molecular Oncology Research Institute, Boston, USA , , Cagil، Emin نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey , , Mengi، Murat نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. , , Ekmekci، Hakan نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey , , Sahmay، Sezai نويسنده Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey , , Nalbantoglu، Burcin نويسنده Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey , , Donma، Mustafa Metin نويسنده Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey ,
Abstract :
Background: Infertility is a problem concerning 10-15% of the individuals in the
fertile period. This study investigated effects of proinflammatory factors as well as
lipid hydroperoxides (LPO) levels upon in vitro fertilization (IVF) success.
Materials and Methods: In this prospective, non-randomized, controlled clinical study,
sera obtained from 26 fertile (group-1), 26 infertile women before (group-2) and after
(group-3) IVF treatment were analyzed. Leptin, leptin receptor, resistin, tumor necrosis
factor-alpha (TNF-?), and C-reactive protein (CRP) were analyzed using enzyme-linked
immunosorbent assay (ELISA). LPO was determined spectrophotometrically. Mann-
Whitney U test, paired samples t test, Wilcoxon signed-rank test as well as Pearson correlation
analysis by SPSS were performed for statistical analysis.
Results: TNF-?, resistin and LPO levels increased (P=0.020, P=0.003, P=0.001, respectively)
in group-3 compared to group-2. A significant increase in LPO was noted both in
group-2 and -3 compared to controls (P=0.000). LPO were higher in non-pregnants than
pregnants in group-2. For pregnants, significant correlations were observed between leptin
and resistin in group-2 and TNF-? and leptin in group-3. None of these correlations
were found for the women, who could not conceive.
Conclusion: LPO, leptin-resistin correlation, associations with TNF-? may be helpful
during the interpretation of IVF success rates.
