Cytokine release from human leukocytes exposed to silorane- and methacrylate-based dental materials

Objectives ne-based dental monomers contain an epoxy functional group. Less is known about the toxicological and inflammatory potential of silorane-based composites. Therefore we compared the release of 24 cytokines from human leukocytes after incubation with silorane-based Filtek™ Silorane (Silo) and methacrylate-based TetricEvo Flow® (TC). s ytes from nine healthy test persons (P) were incubated with Silo or TC for up to 72 h. All 24 h cytokines were quantified with a magnetic bead assay. s timulates the leukocytes to higher release of cytokines when compared to TC. 72 h after beginning the experiment, leukocytes from P6 incubated with Silo secreted more than an 18-fold amount of interleukin (IL)-6 when compared with leukocytes incubated with TC (771.8 vs 42.1 pg/ml). eukocytes from P8 incubated with Silo release up to 14.4 pg/ml IL-2 after 72 h. icance gnificantly higher induction of cytokines with Silo in comparison to TC is test person independent. This indicates a higher sensitization potential for Silo. Because of the cytokine release pattern (especially the release of T-cell dependent IL-2) from leukocytes from P8 after incubation with Silo it is likely that P8 can develop an allergic Type IV sensitization to Silo. Therefore the cytokine release assay is a helpful tool for providing information about possible immunological reactions to dental resins in individual cases as well as for a general risk assessment and comparison between different dental materials.