Author/Authors :
-، - نويسنده Department of Chemical Engineering, Tarbiat Modares University, Tehran, I.R. Iran Tavakol, Moslem , -، - نويسنده Department of Chemical Engineering, Tarbiat Modares University, Tehran, I.R. Iran Vasheghani-Farahani, Ebrahim , -، - نويسنده Department of Nanotechnology, Stem Cell Technology Research Center, Tehran, I.R. Iran
and
Department of Nanotechnology, Stem Cell Technology Research Center, Tehran, I.R. Iran Soleimani, Masoud , -، - نويسنده Department of Food Science and Technology, Faculty of Nutrition and Food Science, Shahid Beheshti University of Medical Science Mohammadifar, Mohammad Amin , -، - نويسنده Department of Chemical Engineering, Tarbiat Modares University, Tehran, I.R. Iran Hashemi-Najafabadi, Sameereh , -، - نويسنده Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, I.R. Iran Hafii, Maryam
Abstract :
Background: The excellent biocompatibility, biodegradability and biological properties of the hydrogels, fabricated using natural polymers, especially polysaccharides, are very advantageous for biomedical applications. Gum tragacanth (GT) is a heterogeneous highly branched anionic polysaccharide, which has been used extensively in food and pharmaceutical industries. Despite, its desirable properties, the potential biomedical applications of this natural gum have not been fully explored. In this study, an enzyme catalyzed in situ forming hydrogel, based on Iranian gum tragacanth (exudate of Astragalus fluccosus) was prepared and characterized for biomedical applications.
Objectives: The main objective of the present study was to explore the feasibility of using tragacanth natural gum as a base for in situ-forming hydrogels in biomedical applications.
Materials and Methods: First, tyramine (TA) was conjugated to the water-soluble part of GT (TGA) using aqueous-phase carbodiimide activation chemistry. Next, in situ forming hydrogel was prepared via an enzyme catalyzed coupling reaction in the presence of horseradish peroxidase (HRP) and H2O2. Gelation time, swelling/degradation behavior and mechanical properties of the hydrogel and cell viability of the encapsulated cells within these hydrogels were investigated.
Results: The gelation time of the hydrogel was less than 30 seconds, which is very desirable for clinical applications. At concentrations ≤ 0.1% (w/v), both GT and TA-TGA showed no toxicity towards human mesenchymal stem cells (hMSCs) and Caco-2 cells. More than 90% of the encapsulated hMSCs in the hydrogels, which were prepared at H2O2 concentrations of less than 15.0 mM, remained viable after 2 hours of incubation.
Conclusions: The TA-TGA conjugate can be gelled enzymatically in the presence of HRP and H2O2. This in situ forming hydrogel might be a desirable candidate for biomedical applications.
