Author/Authors :
Ghaderian ، Sayyed Mohammad Hossein نويسنده Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences & Health Services,Tehran, Iran Ghaderian , Sayyed Mohammad Hossein , Mehrabani Natanzi، Mahboobeh نويسنده Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, IR Iran Mehrabani Natanzi, Mahboobeh , Goudarzvand، Mahdi نويسنده Physiology and Pharmacology Department, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, IR Iran Goudarzvand, Mahdi , Khodaii، Zohreh نويسنده Department of Biochemistry, Nutrition, Medical Genetics, Faculty of Medicine, Alborz University of Medical Sciences & Health Services, Karaj, Iran ,
Abstract :
Prevention of adverse microbial colonization is supposed to be the most important beneficial effect of the gut microflora. The aims of the present study were to compare the effect of co-incubation, pre-incubation and supernatant of sixteen probiotic strains on prevention of enteroinvasive E. coli adhesion. Probiotic strains were added to Caco-2 cells followed by E. coli in pre-incubation assay. Tested strains and enteroinvasive E. coli were added to cell lines at the same time in co-incubation assay. Finally, enteroinvasive E. coli was treated with bacteria free supernatant of test strains then added to cell line in treatment with bacteria free supernatant assay. This study showed that the most effective assays in prevention of pathogen adherence were treatment with bacteria free supernatant and pre-incubation respectively. The effect of probiotic bacteria by-products on pathogen exclusion may be of more importance in protecting the host. Therefore, gut colonization or at least persistent presence of probiotics may be helpful in infection prevention.
