Author/Authors :
Firoozeh، Farzaneh نويسنده Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran , , Shahcheraghi، Fereshteh نويسنده , , Zahraei Salehi، Taghi نويسنده Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, P.O. Box 14155-6453,Tehran, I.R. Iran , , Aslani، Mohammad-Mehdi نويسنده Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran , , Banisaeed، Reihaneh نويسنده Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IR Iran ,
Abstract :
The frequency of multidrug-resistance (MDR) among Salmonella isolates has increased dramatically. Here we report the first case of CTX-M type ß–lactamase producing ciprofloxacin-resistant Salmonella infection as a child in Iran. Antimicrobial resistance profile was determined according to the Clinical and Laboratory Standards Institute. A double disk synergy test was used in the production of extended-spectrum β-lactamases (ESBLs). PCR assays were carried out to detect blaTEM, blaSHV and blaCTX-M beta lactamase genes. The presence of class 1 integrons was investigated by PCR assays. The Salmonella Enteritidis isolate from the child was ciprofloxacin and extended-spectrum cephalosporins resistant and found to carry the blaCTX-M beta-lactamase gene and class 1 integrons, suggests that antimicrobial resistance determinants may be located on the same genetic unit of the class 1 integrons identified here. Resistance to ciprofloxacin and extended-spectrum cephalosporins, the drugs of choice for treating invasive salmonellosis, especially in children is a public health problem.
