Author/Authors :
-، - نويسنده Department of Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, PR China Huang, Weijuan , -، - نويسنده Department of Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, PR China Li, Feifei , -، - نويسنده Department of Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, PR China Liu, Yujing , -، - نويسنده 1-Department of Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, PR China
2-Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China Long, Chunlin
Abstract :
Background: Saffron (Crocus sativus L.) is a common but very expensive herbal medicine. As an important traditional medicine, it has an outstanding effect in treating irregular and painful menstruation. Recently, the over-demand tendency of saffron results in an unusual phenomenon in the medicinal markets. Adulterants and saffron-like substitutes are intentionally mixed into medicinal markets and pharmacies or online stores, affecting drug safety and food quality.Objectives: Our study aimed to identify saffron from its adulterants via DNA barcoding. Materials and Methods: Samples (13 saffron + 4 others containing Carthamus tinctorius or Chrysanthemum x morifolium) obtained from 12 different provinces of China. Through DNA barcoding, samples were compared using three candidate markers, trnH-psbA, rbcL-a and ITS2.Results: trnH-psbA and rbcL-a were capable of distinguishing different accessions. ITS2 could identify samples even at intra-specific level. According to these three barcodes, four samples were identified saffron-like substitutes.Conclusions: The adulterant rate in Chinese markets reaches as high as 33.33% that may cause health risks and further may reduce saffron efficacy once is being used as herbal remedy. In order to make a distinction between C. sativus with other genera as adulterants, DNA barcoding is suggested.
