Author/Authors :
Geranmayeh، Mohammad Hossein نويسنده Section of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. , , Baghbanzadeh، Ali نويسنده Section of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. , , Barin، Abbas نويسنده Department of Clinical Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran Barin, Abbas , Salar-Amoli، Jamileh نويسنده Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran and Toxicology and animal Poisoning Research Center, University of Tehran, Tehran, Iran. , , Dehghan، Mohammad Mehdi نويسنده Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. , , Rahbarghazi، Reza نويسنده Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. , , YOUSEFI-AZARI، HASSAN نويسنده ,
Abstract :
Purpose: Glutamate is a major excitatory neurotransmitter in mammalian central nervous system. Excessive glutamate releasing overactivates its receptors and changes calcium homeostasis that in turn leads to a cascade of intracellular events causing neuronal degeneration. In current study, we used neural stem cells conditioned medium (NSCs-CM) to investigate its neuroprotective effects on glutamate-treated primary cortical neurons. Methods: Embryonic rat primary cortical cultures were exposed to different concentrations of glutamate for 1 hour and then they incubated with NSCs-CM. Subsequently, the amount of cell survival in different glutamate excitotoxic groups were measured after 24 h of incubation by trypan blue exclusion assay and MTT assay. Hoechst and propidium iodide were used for determining apoptotic and necrotic cell death pathways proportion and then the effect of NSCs-CM was investigated on this proportion. Results: NSCs conditioned medium increased viability rate of the primary cortical neurons after glutamate-induced excitotoxicity. Also we found that NSCs-CM provides its neuroprotective effects mainly by decreasing apoptotic cell death rate rather than necrotic cell death rate. Conclusion: The current study shows that adult neural stem cells could exert paracrine neuroprotective effects on cortical neurons following a glutamate neurotoxic insult.
