Comparative Proteomic Profiling of Leishmania tropica: Investiga¬tion of a Case Infected with Simultaneous Cutaneous and Viscero¬tropic Leishmaniasis by 2-Dimentional Electrophoresis and Mass Spectrometry
Hajjaran، Homa نويسنده Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , , Mousavi-Shafaei، Parisa نويسنده , , BURCHMORE، Richard نويسنده Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Science, University of Glasgow, United Kingdom BURCHMORE, Richard , MOHEBALI، Mehdi نويسنده Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran MOHEBALI, Mehdi , MOHAMMADI BAZARGANI، Mitra نويسنده Agriculture institute, Iranian Research organization for Science and Technology, Tehran, Iran MOHAMMADI BAZARGANI, Mitra , Hosseini Salekdeh، Ghasem نويسنده Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran , , KAZEMI-RAD، Elham نويسنده Dept. of Medical Parasitology, Pasteur Institute of Iran, Tehran, Iran KAZEMI-RAD, Elham , KHORAMIZADEH، Mohammad Reza نويسنده Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran AND Dept. of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Iran KHORAMIZADEH, Mohammad Reza
Background: Viscerotropic leishmaniasis caused by Leishmania tropica poses a significant problem in the diagnosis and treatment management. Since differential gene expression is more important in outcome of the infection, we employed proteomic approach to identify potential proteins involved in visceralization of L. tropica.
Methods: The proteomes profiling of L. tropica isolated from cutaneous and visceral tissues of one host were compared by 2-DE/MS proteomics study. Moreover, the transcript level of some identified proteins was confirmed using real-time RT-PCR.
Results: Of the 700 protein spots that were detected reproducibly on each gel, 135 were found to be differentially expressed (P≤ 0.05). Most of responsive proteins in visceral isolate changed in less abundant compared to cutaneous isolate. Among differentially expressed proteins, 56 proteins were confidently identified and classified according to the biological process. The largest groups consist of proteins involved in carbohydrate metabolism and protein synthesis. Most of the identified proteins, which implicated in energy metabolism, cell signaling and virulence were down-regulated, whereas some proteins that have a role in protein folding, antioxidant defense and proteolysis were up-regulated in visceral form. Moreover, the transcript level of some identified proteins such as co-chaperon was confirmed using real-time RT-PCR.
Conclusion: L. tropica probably uses different mechanisms for survival and multiplication in viscera to establish viscerotropic leishmaniasis. The current study provides some clues into the mechanisms underlying the dissemination of L. tropica.