Title of article :
Effect of Alpha-Hederin, the active constituent of Nigella sativa, on miRNA-126, IL-13 mRNA levels and inflammation of lungs in ovalbumin-sensitized male rats
Author/Authors :
-، - نويسنده Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Fallahi, Maryam , -، - نويسنده Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Keyhanmanesh, Rana , -، - نويسنده Department of molecular medicine, School of advanced medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran Khamaneh, Amir Mahdi , -، - نويسنده Department of basic sciences, College of veterinary medicine, Tabriz branch, Islamic Azad University, Tabriz, Iran Ebrahimi Saadatlou, Mohammad Ali , -، - نويسنده Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran Saadat, Saeideh , -، - نويسنده Tuberculosis and lung diseases research center, Tabriz University of Medical Sciences, Tabriz, Iran Ebrahimi, Hadi
Abstract :
Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism. Materials and methods: Forty rats were randomly grouped in 4 groups; control (C), sensitized (S), sensitized pretreated groups with thymoquinone (3 mg/kg i.p., S+TQ) and alpha-hederin (0.02 mg/kg i.p., S+AH). Levels of IL-13 mRNA and miRNA-126 in lung tissue and its pathological changes in each group were assessed. Results: Elevated levels of miRNA-126, IL-13 mRNA and pathological changes were observed in the sensitized group compared to the control group (pConclusion: The results suggested that alpha-hederin had preventive effect on sensitized rats like thymoquinone. It may intervene in miRNA-126 expression, which consequently could interfere with IL-13 secretion pathway leading to a reduction in inflammatory responses.
