Author/Authors :
Culha، Gülnaz نويسنده Department of Microbiology and Clinical Microbiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey. Culha, Gülnaz , Akyar، Isin نويسنده Department of Medical Microbiology, School of Medicine, Acibadem University, Istanbul, Turkey. Akyar, Isin , Yildiz Zeyrek، Fadile نويسنده Department of Microbiology and Clinical Microbiology, School of Medicine, Harran University, Sanliurfa, Turkey. Yildiz Zeyrek, Fadile , Kurt، ?zgür نويسنده Department of Medical Microbiology, School of Medicine, Acibadem University, Istanbul, Turkey. Kurt, ?zgür , Gündüz، Cumhur نويسنده Department of Medical Biology, School of Medicine, Ege University, Izmir, Turkey. Gündüz, Cumhur , ?zensoy T?z، Seray نويسنده Department of Medical Parasitology, School of Medicine, Ege University, Izmir, Turkey. ?zensoy T?z, Seray , ?stan، Ipek نويسنده Vocational School of Health Sciences, Celal Bayar University, Manisa, Turkey. ?stan, Ipek , Cavus، Ibrahim نويسنده Department of Medical Parasitology, School of Medicine, Celal Bayar University, Manisa, Turkey. Cavus, Ibrahim , Gülkan، Burcu نويسنده Department of Microbiology and Clinical Microbiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey. Gülkan, Burcu , Kocag?z، Tanil نويسنده Department of Medical Microbiology, School of Medicine, Acibadem University, Istanbul, Turkey. Kocag?z, Tanil , ?zbel، Yusuf نويسنده Department of Medical Parasitology, School of Medicine, Ege University, Izmir, Turkey. ?zbel, Yusuf , ?zbilgin، Ahmet نويسنده Department of Medical Parasitology, School of Medicine, Celal Bayar University, Manisa, Turkey. ?zbilgin, Ahmet
Abstract :
Background: Cutaneous leishmaniasis (CL) is endemic in Southeastern Anatolia, mainly in Sanliurfa and Hatay provinces, and the causative agents are mostly Leishmania tropica and less frequently L. infantum. Here, we report the first MAL-DI-TOF analyses of Leishmania promastigotes obtained from the cultures of two CL cases from Osmaniye and Hatay provinces who were initially diagnosed by microscopy, culture and identified as L. infantum with Real-Time PCR (RT-PCR).
Methods: Samples obtained from the skin lesions of patients were initially stained with Giemsa and cultivated in NNN medium. Examination of the smears and cultures revealed Leishmania amastigotes and promastigotes, respectively. The promastigotes (MHOM/TR/2012/CBU15 and MHOM/TR/2012/MK05) ob-tained from the cultures of both patients were used for RT-PCR targeting the ITS-1 region in the SSU of rRNA. The reference strains of four Leishmania spe-cies (L. infantum, L. donovani, L. tropica and L. major) were initially assessed with MALDI-TOF and their data were added to MALDI-TOF Biotyper Library.
Results: Both RT-PCR and MALDI-TOF analyses indicated that the causative agent in both patient samples was L. infantum.
Conclusion: Despite disadvantages such as requirement of culture fluid with nothing but promastigotes and high cost, MALDI-TOF analysis may be a fast, sensitive and specific diagnostic tool in especially large-scale research studies, where the cost declines, relatively.
