Application of the Reverse Line Blot Assay for the Molecular Detection of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan.
Iqbal، F نويسنده Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University Multan 60800, Pakistan. Iqbal, F , Khattak، Rm نويسنده Department of Zoology, Kohat University of Science and Technology, Kohat, Pakistan Khattak, Rm , Ozubek، S نويسنده Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey Ozubek, S , Khattak، Mnk نويسنده Department of Zoology, University of Hazara, Mansehra, Pakistan Khattak, Mnk , Rasul، A نويسنده Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130021, China Rasul, A , Aktas، M نويسنده Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey Aktas, M
Background: The present study was designed to detect the presence of tick-borne parasites (Theileria and Babesia spp.) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan.
Methods: Reverse line blot (RLB) assay was applied for the parasitic detection by the amplification of hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene. A membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified PCR products.
Results: Parasites were detected in 16% of the ruminant blood samples under study. Two Theileria species, T. lestoquardi and T. ovis, were identified in samples. 25, of the total 32, infected animals were from Khyber Pukhtoon Khwa.
Conclusion: Sheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats (P > 0.001). Risk factor analysis revealed that male (P = 0.03), ani-mals infested by ticks (P = 0.03) and herd composed of sheep only (P = 0.001) were more infected by blood parasites.