Author/Authors :
Ghanbari-Niaki، Abbass نويسنده , , Ghanbari Abarghooi، Safieyh نويسنده Department of Exercise Biochemistry, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Baboulsar, IR Iran Ghanbari Abarghooi, Safieyh , Rahbarizadeh، Fatemeh نويسنده , , Zare-Kookandeh، Navabeh نويسنده Exercise Biochemistry Branch, Faculty of Physical Education and Sports Science, University of Mazandaran, Babolsar, IR Iran , , Gholizadeh، Monireh نويسنده Department of Exercise Biochemistry, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Baboulsar, IR Iran Gholizadeh, Monireh , Roudbari، Fatemeh نويسنده Department of Molecular and Cell Biology, University of Mazandaran, Babolsar, IR Iran , , Zare-Kookandeh، Asghar نويسنده Tehran Heart Center ,
Abstract :
Heart as a high metabolic and aerobic tissue is consuming lipid as a fuel for its energy provision at rest during light and moderate exercise, except when lactate level is higher in blood circulation. It has been shown that any type of regular exercise and crataegus species would improve cardiovascular function and minimizes several risk factors via stimulating lipid metabolism by acting on enzymes and genes expression such as ABCA1 and PPAR α which are involving in this process. Twenty Wistar male rats (4-6 weeks old, 140-173 g weight) were used. Animals were randomly classified into training (n = 10) and control (n = 10) groups and then divided into saline-control (SC), saline-training (ST), Crataegus-Pentaegyna -control (CPC), and Crataegus-Pentaegyna -training (CPT) groups. Training groups have performed a high-intensity running program (at 34 m/min (0% grade), 60 min/day, 5 days/week) on a motor-driven treadmill for eight weeks. Animals were orally fed with Crataegus-Pentaegyna extraction (500mg/kg) and saline solution for six weeks. Seventy- two hours after the last training session, rats were sacrificed, hearts were excised, cleaned and immediately frozen in liquid nitrogen and stored at -80 °C until RNA extraction. Plasma also was collected for plasma variable measurements. Statistical analysis was performed using a two way analysis of variance, and significance was accepted at P < 0.05. A non-significant (P < 0.4, P < 0.79, respectively) increase in ABCA1 and PPAR α genes expression was accompanied by a significant (P < 0.01, P < 0.04, P < 0.04, respectively) reduction in TC, TG, and VLDL-C levels in Crataegus-Pentaegyna groups. Our findings show that a high intensity treadmill running was able to express ABCA1 and PPAR α in rat heart. Data also possibly indicate that the Crataeguse-Pentaegyna supplementation solely could mimic training effect on the mentioned genes and lipid profiles via different mechanism(s).
