Carbon disulfide is used predominantly in the manufacture. It has affects the nervous system. In this study, the applicability of SPME as a passive sampler for determination of carbon disulphide in air was studied. Effect of sampler and environmental parameters on uptake of Carbon disulphide was studied as well. Four fibers were tested to select the best sampler for determine carbon disulfide in ambient air. A standard generation chamber was built in the laboratory and was used to test the SPME. Analysis SPME samples were carried out by a gas chromatography-Mass spectrometry and results were compared with data obtained with National Institute for Occupational Safety and Health (NIOSH) method 1600. Polymethylsiloxane-carboxen (PDMS/CAR) showed the most effective stationary phase material for sorbing BTEX among other materials (polyacrylate, PDMS, PDMS/divinylbenzene). Its linearity range in exposed mode was less than 10 minutes but with its retracted mode application, its linearity increased up to 8 hours. Temperature had not linear effect on uptake of pollutant in temperatures lower than 25, it has positive effect and above this range it has negative effect. Relative humidity had negative effect on mass loaded on fiber. Velocity in range of static to 0.5 m/s had no significant effect. The precision of the method was 4.18% relative standard deviation (RSD). The detection limit for carbon disulfide in the GC/MS system in SIM mode was 6.7 ng per sample. SPME is a good alternative for sampling of carbon disulfide in air. However, for the situations in high humidity values it should be used with care.