Important factor in the prevention of industrial accidents involve of the ability of employees to maintain awareness of the work situation, understand the information it holds, and predict how situations will develop. In this study, we examined the role of fatigue and work overload in predicting work situation awareness among workers. The current study was a cross-sectional study. The sample consisting of 180 employees in National Petrochemical Company in 2014 was selected according to the stratified random sampling method and responded to questionnaires about demography characteristics, work situation awareness, fatigue and work overload. The data were analyzed by correlation techniques and stepwise regression. There was internal correlation among fatigue, work overload and work situation awareness. In addition, the results of stepwise regression analysis revealed that fatigue and work overload significantly predicted, respectively, almost 33% and 14% of variances of work situation awareness among workers. Fatigue and work overload can predict work situation awareness. Therefore, considering these variables can be important to promoting the awareness of work situation among workers.