Author/Authors :
Moridi، Heresh نويسنده Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran , , Karimi، Jamshid نويسنده of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. , , Sheikh، Nasrin نويسنده , , Goodarzi، Mohammad Taghi نويسنده , , Saidijam ، Massoud نويسنده Department of Genetics and Molecular Medicine, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , , Yadegarazari، Reza نويسنده Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Yadegarazari, Reza , Khazaei، Mohammad Rasool نويسنده Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran , , Khodadadi، Iraj نويسنده , , Tavilani، Heidar نويسنده Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. , , Piri، Hossein نويسنده University of Bonab , , Asadi، Soheila نويسنده Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; , , Zarei، Sadegh نويسنده Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; , , Rezaei، Azam نويسنده Islamic Azad University of Arsanjan, Arsanjan, I. R. Iran ,
Abstract :
Millions of people in the world have diabetes mellitus and its prevalence is growing. Oxidative stress, advanced glycation end-products (AGEs) and receptor for advanced glycation end-products (RAGE) play key role in the pathogenesis of diabetes. New and safe strategies of remedy are needed for this disease. We hypothesized that resveratrol may exert a renal protective effect on diabetic rats. Male rats with diabetes were treated with or without resveratrol as 1, 5, 10 mg/kg of body weight for 30 days. The total AGEs and malondialdehyde levels in kidney tissues were determined by spectrofluorimetric method and the insulin level was assayed using ELISA. The total antioxidant capacity contents in kidney and the glucose in plasma were measured by a colorimetric assay. The expression of RAGE was assayed in kidneys of all animals using quantitative PCR. In resveratrol-treated rats with diabetes, malondialdehyde levels, plasma glucose and expression of RAGE were significantly reduced compared with the untreated group. Moreover, the total antioxidant and insulin levels significantly increased in treated rats. There was no significant difference in the AGEs contents among all the groups. These results revealed that resveratrol has beneficial effects on kidney by extenuating the oxidative stress and down-regulation of RAGE expression.
