Author/Authors :
Kermanshahani، Mohammad نويسنده Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. , , Naderi، Roohangiz Naderi نويسنده , , Fattahi، Reza نويسنده Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran , , Khalighi، Ahmad نويسنده Faculty of Agriculture and Natural Sources, Science and Research Branch, Islamic Azad University, Tehran, I. R. Iran ,
Abstract :
Low seed yield is a limiting factor for cross breeding and hybrid
seed production in cyclamen. This study was performed to investigate
pollen germination and its relation to cross-pollination success and fruit
set in this plant. In order to achieve a high level of pollen germination, the
effect of different concentrations of chemical compounds were examined
on in vitro pollen germination of cyclamen in modified Brewbaker and
Kwack medium, containing sucrose (10 and 20%), calcium nitrate (0, 200
and 300 mg l-1), and boric acid (0, 100 and 200mg l-1) at two pH levels (5.5
and 6.5). Maximum pollen germination was obtained in media containing
higher concentration of calcium and boron regardless of sucrose concentration
and pH level. Pollen germination percentage was genotype-dependent.
Cross-pollination was performed among four different genotypes characterized
by various pollen germination percentages. There was a direct correlation
between cross-pollination success and pollen germination percentage.
Genotypes with 30% higher pollen germination led to 10% increase in
fruit set.
