Author/Authors :
Habibi، GR نويسنده Parasite Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, Iran Habibi, GR , Imani، AR نويسنده Veterinary College, Islamic Azad University, Karaj, Iran Imani, AR , Gholami، M.R. نويسنده , , Hablolvarid، M.H. نويسنده , , Behroozikhah، AM نويسنده Brucellosis Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, Iran Behroozikhah, AM , Lotfi، M نويسنده Quality Control of Biological Products group, Razi Vaccine and Serum Research Institute, Karaj, Iran Lotfi, M , Kamalzade، M نويسنده Quality Control of Biological Products group, Razi Vaccine and Serum Research Institute, Karaj, Iran Kamalzade, M , Najjar، E نويسنده Veterinary Organization of Qazvin Province, Qazvin, Iran Najjar, E , Esmaeil-Nia، K نويسنده Parasite Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, Iran Esmaeil-Nia, K , Bozorgi، S نويسنده Parasite Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, Iran Bozorgi, S
Abstract :
Background: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran.
Methods: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathological observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally.
Results: The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections.
Conclusion: The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes.
