Title of article :
Species Identification of Acanthamoeba Strains Isolated from Patients Referring to Farabi Eye Reference Center Using PCR-RFLP Method
Author/Authors :
Ghamilouie، Mir Mostafa نويسنده Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran , , Valadkhani، Zarrintaj نويسنده Department of parasitology, Pasteur Institute of Iran, Tehran, IR Iran , , Khoshzaban، Fariba نويسنده Department of Parasitology and Mycology, Faculty of Medical Sciences, Shahed University, Tehran, IR Iran ,
Issue Information :
فصلنامه با شماره پیاپی 0 سال 2014
Pages :
5
From page :
125
To page :
129
Abstract :
Introduction: Acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. Infection with Acanthamoeba spp. poses threat to human health, such as, Acanthamoeba keratitis (AK) that is a vision-threatening infection of the cornea. This study aimed to identify the species of Acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Methods: Acanthamoeba isolates investigated in this cross-sectional study, were collected from patients referring to Farabi Eye Reference Center. All 10 isolates were subjected to species identification using DNA based method. BspLI (NlaIV) and HpyCH4IV restriction enzymes were used to categorize the PCR amplified DNA by PCR-RFLP method. Results: Six samples were identified as Acanthamoeba palestinensis and 4 isolates as Acanthamoeba culbertsoni, which implies that all the isolates belong to pathogenic strains of Acanthamoeba. Conclusion: Acanthamoeba can enter the corneal tissue and survive in the eye, which results in AK. To the authorsʹ knowledge, no study is available on species identification of this genus using these enzymes and technique. This is the first time in Iran that Acanthamoeba isolates are subjected to species identification using PCR-RFLP method.
Journal title :
Journal of Medical Microbiology and Infectious Diseases
Serial Year :
2014
Journal title :
Journal of Medical Microbiology and Infectious Diseases
Record number :
2384831
Link To Document :
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