Indirect Shoot Regeneration in Anthurium andreanum ‘Clisto’ from Leaf Explant

Anthurium is commercially produced as cut flowers and potted plants and known for its colorful spathes. It is traditionally propagated by stem cutting and suckers, so micropropagation is an alternative production method. In this experiment we carried out shoot regeneration in Anthurium andreanum ‘Clisto’. The leaf explant was used and after sterilization, they cultured on two different media (half-strength MS and Nitsch’s media) which supplemented with different compositions of 2,4-D (0, 0.25, 0.5 and 0.75 mg/L) and BA (0, 0.5, 1 and 1.5 mg/L). After three months callus size and percentage of explant that produced callus were measured and callus transferred to MS media with different concentrations of BA (0, 0.25, 0.5 and 0.75 mg/L). After 6 months, number of shoot, shoot length and leaf number was recorded. The highest callus proliferation was obtained in Nitsch’ media supplemented with 0.25 mg/L 2,4-D and 0.25 or 0.5 mg/L BA. The callus was creamy color and compact. The highest shoot regeneration was observed in media with 0.75 mg/L BA. We cloud achieved 31 shoot per callus segment in the best treatment. Rooting was easily performed in peat: perlite (1:2) pots and they well acclimated and transferred to greenhouse and 99% plants were survived.