Author/Authors :
Mahdavi، Mehdi نويسنده Department of Immunology, Pasteur Institute of Iran, Tehran, Iran , , Ebtekar، Massoumeh نويسنده , , Mohammad Hassan، Zuhair نويسنده , , Faezi، Sobhan نويسنده Department of Bacteriology, Pasteur Institute of Iran, Tehran, IR Iran , , Khorram Khorshid، Hamidreza نويسنده Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. Khorram Khorshid, Hamidreza , Taghizadeh، Morteza نويسنده Department of Influenza, Razi Vaccine and Serum Research Institute, Alborz, Iran. , , Azadmanesh، Keyhan نويسنده Department of Virology, Pasteur Institute of Iran ,
Abstract :
Memory formation is the most important aspect of a vaccine which can guarantee long-lasting immunity and
protection. The main aim of the present study was to evaluate the memory immune responses after immunization
with a mini vaccine. Mice were immunized with human immunodeficiency virus-1 P24-Nef fusion peptide and
then cellular and humoral immune responses were evaluated. In order to determine long-lived memory, immune
responses were monitored for 20 weeks after final immunization. The results showed that the candidate vaccine
induced proliferation and cytotoxic T lymphocyte responses and shifted cytokine patterns to T helper-1 profile.
Evaluation of humoral immune responses also showed an increase in total peptide specific-IgG titer and a shift to
IgG2a humoral response. Monitoring of immune responses at weeks 4, 12 and 20 after last immunization
showed that immunologic parameters have been sustained for 20 weeks. Our findings support the notion that
long-lived memory responses were achieved using a mini vaccine immunization.
