Nowadays, using public transportation vehicles like metro in large cities are of significance, and exposure to whole-body vibration for the metro passengers is one of the physical contaminations. This paper is conducted with the aim of evaluating the passengers’ convenience and health both in sitting and standing conditions. The present study was done on the passengers of lines 1, 2, and 4 of Tehran metro with the use of an oscillator and a SVAN958 analyzer of SVSNTEK Co., based on the measurement methods and guidelines of ISO 2631-1, 4. The average amounts of RMS acceleration while sitting was 1.1 m/s2 with the standard deviation of 0.14 and while standing was 0.91 m/s2 with the standard deviation of 0.05; estimated VDV amount while sitting and standing equaled 6.72 m/s2 with the standard deviation of 1.26 and 6.48 m/s1.75 with the standard deviation of 0.96. Calculated RMS amounts generally fluctuated between level 3 (fairly unpleasant) and level 4 (unpleasant) of convenience evaluation scale, and total calculated VDV amounts for the sitting passengers was 1.1 m/s1.75 , being more than the perceived dose of standing passengers.