Author/Authors :
Jasemizad، Tahereh نويسنده Department of Environmental Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Mokhtari، Mehdi نويسنده Department of Environmental Health Engineering, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Zandi، Hengameh نويسنده Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Zandi, Hengameh , Shahriari، Taher نويسنده Department of Environmental Health Engineering, School of Public Health AND Social Determinants of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran , , Sahlabadi، Fatemeh نويسنده Department of Environmental Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Montazeri، Akram نويسنده Environmental Health Expert, Shahid Sadoughi Accidents Burns Hospital, Yazd, Iran , , Dehghani Tafti، Arefeh نويسنده MSc Student in Biostatistics, Isfahan University of Medical University, Isfahan, Iran ,
Abstract :
Background and Purpose: The burn unit is a suitable environment for growing of bacteria such
as Pseudomonas, Acinetobacter, and Staphylococcus that appropriate disinfection can reduce
these pathogens. The aim of this study was to evaluate the effect of different disinfectants on
Gram-negative bacteria isolated from the surface of accidents and burn hospital in Yazd.
Materials and Methods: In this study, 240 samples were randomly collected from different parts of
accidents and burn hospital before and after disinfection. The samples were cultured on blood agar
and Eusion-Metilen-Blue agar media in the Microbiology Laboratory of Medicine School of Shahid
Sadoughi University in Yazd and Colony counting were determined. Identification was done by
biochemical tests after incubation at 37° C for 48 hours. The studied disinfectants were Deconex
50AF, Descoscid, Epimax SC, and Silvosept. At last, data were analyzed with using paired t-test.
Results: The Gram-negative bacteria were isolated from burn unit before disinfection included
Pseudomonas aeruginosa, Escherichia coli, Proteus spp., Klebsiella spp., Acinetobacter spp., and
Enterobacter spp. According to the results, all disinfectants reduced the pollution before and
after disinfection; nevertheless, this reduction at the time of using Epimax SC and Silvosept only
showed a significant difference for P. aeruginosa (P = 0.001 and 0.003) and for E. coli
(P = 0.020 and 0.005), respectively.
Conclusion: All disinfectants were effective on Gram-negative bacteria isolated from surfaces
and had shown a significant difference only between P. aeruginosa and E. coli number before
and after disinfection by Epimax SC and Silvosept. The most effective disinfectant on P.
aeruginosa and E. coli was Epimax SC and Silvosept, respectively.
[Jasemizad T, Mokhtari M, Zandi H, Shahriari T, *Sahlabadi F, Montazeri A, Dehghani Tafti
