Author/Authors :
Ghavipanjeh، F نويسنده Energy Department, Materials and Energy Research Center, Meshkin Dasht, Karaj, IR Iran , , Pazouki، M نويسنده Energy Department, Materials and Energy Research Center, Meshkin Dasht, Karaj, IR Iran , , Ziaei Rad، z نويسنده Energy Department, Materials and Energy Research Center, Meshkin Dasht, Karaj, IR Iran , , Hosseinnia، A نويسنده Energy Department, Materials and Energy Research Center, Meshkin Dasht, Karaj, IR Iran ,
Abstract :
Long-chain alkanes are a major constituent of crude oils and their conversions into
other compounds are of interest depending on the specific application. Here, five
native microbial consortia obtained from petroleum polluted sites were examined for
biological conversion of n-octadecane as a representative of long chain alkanes. The
experiments were implemented in 250 mL flasks containing 0.5 g n-octadecane in 40
mL culture media kept on a shaker at 160 rpm and 30?C for one week. A pure culture
of Psedumonas putida was inoculated at the same condition for comparison.
Amongst the consortia, ABN52 imposed more obvious changes on n-octadecane. The
GC-MS analysis of daily samples showed the appearance of lighter branched
compounds at the first and second days of incubation but disappeared in the
following days. At the end of incubation time up to 20 (w/w%) of the initial substrate
was turned into polyhydroxyalkanoates (PHAs). It also suggested higher activity of
the consortia compared to the pure culture of Psedumonas putida.
