Author/Authors :
Noori، Neda نويسنده , , Vandyosefi، Jalil نويسنده Department of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, IR Iran , , Sabet، Faeze نويسنده Antimicrobial Resistance Research Center, Buali Research Institute, Department of Microbiology and Virology, Medical School, Mashhad University of Medical Sciences, Mashhad, IR Iran , , Ashrafi، Sara نويسنده MSc Student, Department Environmental Health, School of Health, Mazandaran University of Medical Sciences, Sari, Iran , , Ghazvini، Kiarash نويسنده ,
Abstract :
Background: The emergence and rapid spread of metallo-beta-lactamase (MBL) producing Acinetobacter spp. are of great concern worldwide due to limited treatment options. Epidemiologic studies of the causing genes are important for prevention. Methods: In this study, 70 imipenem-resistant Acinetobacter strains were isolated from health care associated infections. These isolates were screened for detection of metallo-beta-lactamase (MBL) using inhibitor potentiated disk diffusion tests with ethylenediaminetetraacetic acid (EDTA). PCR was designed for detection of bla vim and bla imp-1 using specific primers. Results: Among these 70 strains, 50 strains appeared to produce metallo-betalactamase. Three isolates were detected by PCR to carry metallo-beta-lactamase gene bla vim, but bla imp-1 gene was not detected. Conclusion: These findings suggest that in our area other genetic elements are responsible for resistance against metallo-beta-lactams
