Author/Authors :
Haghkhah، Masoud نويسنده Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Derakhshandeh ، Abdollah نويسنده Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Jamshidi ، Reza نويسنده Department of Clinical Sciences, School of Veterinary Medicine, University of Semnan, Semnan, Iran , , Moghiseh ، Asghar نويسنده of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Karimaghaei ، Negar نويسنده DVM Students, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Ayaseh ، Mohammad نويسنده DVM Students, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Mostafaei ، Mohsen نويسنده M.Sc. student of Political sciences, Payam Noor University of Tehran, Iran. ,
Abstract :
Paratuberculosis (John’s disease) is infectious and chronically progressive granulomatous disease which affects domestic and wild ruminants. The causative agent is Mycobacterium avium paratuberculosis (MAP), a slow growing mycobactin dependent acid-fast bacillus. We investigated the detection and frequency of MAP in apparently healthy dromedary and Bactrian camels by insertion sequence 900 (IS900) polymerase chain reaction (PCR) and acid fast staining of fecal samples in Iran. Acid fast staining results showed that 6/50 (12.0%) samples of dromedary camels and 4/26 (15.3%) samples of Bactrian camels were suspected to MAP. Although the percentage of positivity for PCR assay of fecal dromedary camel was 8.0%, no bands corresponding to MAP detect in all samples of Bactrian camels. In conclusion, Although the incidence of MAP infection was low, further studies should be conducted to get more information on MAP infection in camel population, especially in areas where camels are close to other ruminants such as dairy cow, sheep and goat.
