Author/Authors :
Habibi، Fatemeh نويسنده Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. , , Ghadiri Soufi، Farhad نويسنده Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, Bandar-Abbas, Iran. , , Ghiasi، Rafighe نويسنده Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran. , , Khamaneh، Amir Mahdi نويسنده Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran. , , Alipour، Mohammad Reza نويسنده Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran ,
Abstract :
Purpose: The purpose of the present study is to evaluate the expression of miR-146a gene, its adaptor genes (TRAF6, NF-KB, and IRAK1), and possible changes in the cellular signaling pathway in diabetic hippocampus tissue. Methods: Male SpragueDawley rats are randomly selected and divided into control and diabetic (n=6) groups. Diabetes induced by the single-dose injection of nicotinamide [110 mg/kg, (i.p.)], 15 min before streptozotocin (50 mg/kg; i.p.) in 12-h fasted rats. The rats are kept at the laboratory for two months. After anaesthetization, hippocampus of the rats was removed in order to measure the expression of miR-146a, NFK-B, IRAK1, and TRAF6 genes using real-time PCR and activity of NF-KB as well as amount of apoptosis rate using ELISA. Results: The results indicated a reduction in expression of miR-146a and an increase in expression of IRAK1, NF-KB, and TRAF6 genes in the hippocampus of diabetic rats compared to control. Also it reveals an increase in the activity of NF-KB and apoptosis rate in the hippocampus of diabetic rats. Conclusion: Our results report the probability that reduction of miR-146a expression in the negative feedback loop between miR-146a and NF-KB increases NF-kB expression and thus intensifies inflammation and apoptosis in hippocampus.
