Author/Authors :
Nazemalhosseini Mojarad، Ehsan نويسنده Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran , , Kashfi، Seyed Mohammad Hossein نويسنده 1Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Mirtalebi، Hanieh نويسنده Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , , Almasi، Shohre نويسنده Gastroenterology and Liver Disease Research Center,
Research Institute for Gastroenterology and Liver Diseases, Shahid
Beheshti University of Medical Sciences, Tehran, IR
Iran , , Chaleshi، Vahid نويسنده 1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran , , Kishani Farahani، Roya , نويسنده Basic and Molecular Epidemiology of Gastroenterology Disorders Research Center, Shahid Beheshti University of Medical , , Tarban، Peyman نويسنده Basic and Molecular Epidemiology of Gastroenterology
Disorders Research Center, Research Institute for Gastroenterology
and Liver Diseases, Shahid Beheshti University of Medical Sciences,
Tehran, IR Iran , , Molaei، Mahsa نويسنده , , Zali، Mohammad Reza نويسنده Department of Celiac Disease, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran , , J.K. Kuppen، Peter نويسنده Department of Surgery, Leiden University Medical Center, Leiden, ,
Abstract :
Beta catenin plays a key role in cancer tumorigenesis. However, its prognostic significance in patients with colorectal cancer (CRC) remains controversial. It has been demonstrated that 90% of all tumors have a mutation in individual components of multiple oncogenes in Wnt/B-catenin pathway. Accumulation of nuclear B-catenin in cytoplasm leads to uncontrolled cell proliferation. Thus, nuclear B-catenin accumulation may be a valuable biomarker associated with invasion, metastasis and poor prognosis of CRC. In this study the prognostic value of beta catenin expression in 165 Iranian CRC patients was evaluated. In this cross sectional retrospective study immunohistochemistry analyses of formalin-fixed paraffin-embedded (FFPE) tumor tissues were performed to characterize the expression of nuclear B-catenin in a series of 165 Iranian patients with colorectal carcinoma. Heat-induced antigen retrieval using the microwave method was applied for all staining procedures. Staining was scored independently by two observers, and a high level of concordance (90%) was achieved. Statistical analysis was done using the SPSS software for Windows, version 13.0.0 (SPSS Inc., Chicago, IL). Two-tailed P < 0.05 was considered statistically significant. The patients consisted of 85 males and 80 females. Eighty-eight patients had primary tumor of the rectum and sigmoid, while 77 patients had primary tumor of the colon. The mean period of follow-up was 47.2 ± 10 months and the median period of follow-up was 38 months (range 6 - 58) for each patient. Of 165 tumors, 32 tumors (19.39 %) showed expression of B-catenin and 133 (80.6 %) were negative for B-catenin expression. Based on our findings the distribution of Microsatellite Instability (MSI) status differed between patients with nuclear B-catenin positive and negative tumors and this difference was significant (P = 0.001). Patients with nuclear B-catenin positive expression profile were found to be younger than patients with negative nuclear B-catenin expression (P = 0.010). Univariate and multivariate analysis showed that tumors with B-catenin expression had a poorer prognosis compared to tumors without B-catenin expression. According to our findings, the distribution of nuclear b-catenin expression is a poor prognostic marker in patients with colon cancer
